yitF [2019-06-19 11:17:37]

similar to mandelate racemase

locus: BSU10970

pI: 7.53

mw: 41.89 kDa

protein length: 371 aa

gene length: 1116 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 1,174,861 → 1,175,976

The protein

Protein family

• mandelate racemase/muconate lactonizing enzyme family (according to Swiss-Prot)

Structure

• [PDB|2GDQ]

Expression and Regulation

Operons

• genes: [gene|BA1A7ECAE0B5690F6E6A67785BAB18628701E2A2|yitG]-[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]
  description: [Pubmed|16497325]

  sigma factors

  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|16497325,15699190], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed during sporulation in the forespore ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]) [Pubmed|16497325,15699190]

Biological materials

Mutant

• MGNA-B180 (yitF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1179 NBRP B. subtilis, Japan]
• BKE10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG, downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA
• BKK10970 (Δ[gene|F1A84EC28BE0E306356A331FD362E3CDAE49A3AB|yitF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10970 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGTACAATTTTCACACTGG, downstream forward: _UP4_TAAGCTGGGCCATTTCTTTA

References

• 16497325,15699190